In order to study the content characteristics of available microelements in typical tobacco-planting soils of Shandong Province, soil samples were collected from 6 main tobacco-planting regions, i.e., Linqu, Mengyin, Zhucheng, Feixian, Wulian and Juxian and the available contents of Fe, Mn, Cu and Zn in the samples were analyzed. The results showed that: 1) the contents of available Fe and Mn in the plough horizon were plentiful, while those of Cu and Zn were moderate and insufficient respectively; 2) available Fe, Mn and Zn were obviously accumulated in plough horizon while available Cu distributed homogeneously in the profile; 3) available Fe, Mn, Cu and Zn in soils derived from calcareous purple sandstone were significantly lower than from other parent materials; 4) significantly negative correlations were observed between the contents of available Fe and Mn and soil pH while a significantly positive correlation found between soil available Cu, Zn and soil organic matter.