The matched fertilization of N, P and K that affect seed corn’s nutrient content of N, P and K absorption and output was studied through field experiment. The results showed that fertilization promoted obviously dry biomass accumulation of seed corn. Increasing N and P fertilization could improve the accumulation of dry matter of plants, but the accumulation of dry matter reduced when N was excessive. Reasonable collocation of fertilization of N, P and K promoted the coordinative supply of nutrients proportion and significantly increased nutrient content, nutrient uptake and nutrient harvest index in each organ of seed corn. These results provided a solid foundation for increase of maize yield. Reasonable collocation of fertilization of N, P and K can increase seed corn’s production obviously. Yield-increasing effect of P was less obvious than that of N. For every 100 kg production of corn seed, the optimal uptake of N, P and K respectively was N: 2.25 kg, P2O5: 0.60 kg and K2O: 2.40 kg, and the recommended fertilization was N 360 kg/hm2, P2O5 180 kg/hm2 and K2O 90 kg/hm2.