In this paper, the riparian zone of Bortala River and Jinghe River of Ebinur Lake wetland was selected as a study area, soil available N content was measured and the spatial variation and distribution characteristics of soil available N in the study area were discussed by the method of descriptive statistics, statistical methods and spatial analysis. The results showed that: the spatial correlation of soil available N in the depths 0–20cm, 20–40cm and 60–80cm were moderately dependent, with the Nugget/Sill of 47.60%,、49.98% and 58.21%, respectively; soil available N in the depths of 40–60cm and 80–100cm were highly dependent, with the Nugget/Sill of 3.64% and 22.69%, respectively; within the range of 0–100cm soil depth, the average value of soil available N in each layer (0–20cm, 20–40cm, 40–60cm, 60–80cm and 80–100cm) were 49.16 mg/kg, 37.26 mg/kg, 30.22 mg/kg, 20.35 mg/kg and 17.80 mg/kg, respectively; the frequency of soil available N content in study area mostly ranged between 15–20 mg/kg, which showed the overall level of soil available N content was low, belonging to extremely deficient level; soil available N decreased with the increase of soil depth. Horizontal distribution rules of soil available N content was: Bortala River＞Jinghe River and eastern region of Jinghe River＞central area between Bortala and Jinghe Rivers.