The soil nutrient contents in 355 soil samples of tobacco-planting area in Luoping of Yunnan were surveyed in 2008 and 2014 in order to improve the targeted fertilization. The soil fertility suitability were comprehensive evaluated by soil fertility index (SFI value) and the spatial and temporal variations of soil nutrient contents were studied. The results indicated that: 1) soil pH, rapidly available P, rapidly available K, available Zn and available B contents were at an appropriate level while organic matter, hydrolytic N, available Mg and water-soluble Cl– contents were higher. 2) The integrated fertility index of most tobacco-planting area in Luoping was in better and medium level, the average SFI value was 0.762 9 and soil fertility was in good condition. 3) The soil fertility suitability index in different areas was: Dashuijing>Banqiao>Jiulong>Luoxiong>A’gang>Majie> Zhongshan>Jiujiwu>Laochang>Fule. 4) From 2008 to 2014, the organic matter content reduced, while the pH value, content of hydrolytic N, rapidly available P, rapidly available K, available Zn, available Mg, available B, water-soluble Cl– and SFI value increased.