采用Brad-ford染色法研究了南京市5种典型利用方式土壤不同土层中 (0 ~ 10、10 ~ 20、20 ~ 40 cm)球囊霉素的含量。结果表明：土壤中总球囊霉素含量为1.96 ~ 3.12 mg/g，占土壤有机碳的12.5% ~ 29.0%，所占比例随土壤有机碳含量的增加而降低。林地和草地土壤中球囊霉素和有机碳的含量均高于3种耕作土壤 (水稻田、茶园土和菜园土)。随着土层深度(0 ~ 40 cm)的增加，5种不同利用方式土壤中总球囊霉素和有机碳的含量均减小；与其他土层相比，0 ~ 10 cm土层总球囊霉素和有机碳含量均最大。耕作土壤中易提取球囊霉素更易于向总球囊霉素转化。发现5种土地利用方式下土壤中总球囊霉素含量与土壤有机碳含量极显著正相关，与土壤pH显著负相关；易提取球囊霉素与土壤有机碳含量极显著负相关。总球囊霉素和易提取球囊霉素可作为评价土壤丛枝菌根真菌活性和土壤质量的重要指标。

In this paper, the contents of total glomalin-related soil protein (T-GRSP) and easily extracted glomalin-related soil protein (EE-GRSP) were determined in three depths (0–10, 10–20 and 20–40 cm) of five soils under different land use types by using Bradford staining method. It was found that the T-GRSP contents of all soil samples ranged from 1.96 to 3.12 mg/g, accounting for 12.5%–29.0% of the SOC contents. The percentages of T-GRSP to SOC were decreased with the increase of SOC content. The GRSP and SOC contents in three soils, including tea, paddy, and vegetable soil, under artificial land use types were significantly lower than those in the forestland and grassland. The GRSP and SOC contents in test five soils decreased with increase of soil depth, and the highest values were observed in soil surface layer (0–10 cm). The content of T-GRSP was positively and significantly correlated with the SOC content, while negatively with soil pH value. A negatively significant correlation of EE-GRSP with SOC was also observed. It was suggested that T-GRSP and EE-GRSP can be used as the indicators to evaluate the AMF activity and soil quality.

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国家自然科学基金项目(21477056，41171380)和江苏省杰出青年基金项目(BK20130030)资助