Two solo-tracer infiltration experiments and two multiple-tracer infiltration experiments were conducted in natural undisturbed soil in order to investigate the existence and penetrating depths of macropores and the effects of macropores on the infiltration of applied tracer solutions. The results revealed that full-penetrated macropores (i.e. macropores penetrated the whole infiltrated soil layer) were occurred in Plot 1 (solo-tracer infiltration experiment) and partial-penetrated macropores (i.e. macropores penetrated only part of the infiltrated soil layer) occurred in Plot 2 (multiple-tracer infiltration experiment), while no macopores occurred in Plot 2 (solo-tracer infiltration experiment) and 4 (multiple-tracer infiltration experiment). The tracer solution transported much more rapidly in the full-penetrated macropores to the deeper soil layer in Plot 1, and the transportability of full-penetrated macropores on applied tracer solutions did not change much with the applying sequence of tracer solutions. On the contrary, the partial-penetrated macropores in Plot 3 were much more powerful in transporting the tracer solution applied in the second step while were less powerful in transporting the tracer solutions applied in the first and third steps.