Soil was progressively separated into different particle sizes and into heavy fractions of organic mineral complex by using combination physic-chemical methods. The distribution characteristics of copper (Cu), zinc (Zn) and cadmium (Cd) combined in the different humus fractions and different particle fractions were determined after the application of sewage sludge fertilizers (SSF). The results showed that soil heavy metal concentrations increased with the particle size decrease, but application of SSF increased the proportion of heavy metals in fine particle, whose content increased as the concentration of heavy metal in sludge increased. SSF allocation increased the proportion of loosely combined humus in different particles, while declining the tightly combined humus. SSF addition increased the proportion of Cu, Zn and Cd in loosely combined humus of fine particles, while decreasing the proportion of those metallic elements in tightly combined humus. In the silt fraction, the proportion of Cu and Cd in loosely combined humus was increased while those metallic elements’ proportion in tightly combined humus was declined. The proportion of Cu, Zn and Cd of variously combined humus in both fine silt sand and colloidal particles did not change significantly. The proportion of heavy metals was correlated with the proportion of different combined humus.