The evaluation units in field scale and 2 642 soil sampling sites which located in the area of Huaishang district of Bengbu, Anhui Province were obtained using remote sensing interpreting and GPS-based sampling point arrangement. In the meantime, a relatively scientific evaluation model has been established using the nutrient status of arable lands, physicochemical properties, geographical condition, management situation and other 17 factors as parameters and assisted by spatial analysis of GIS, DELPH method, AHP and fuzzy assessment. Accumulation curve method was used to determine the fertility level of the evaluation limits, the 20000 evaluation units of the upper areas were divided into 5 classes. The first and second gradations of croplands were mainly distributed in Caolaoji Town and the third and fifth gradations of croplands were distributed in Meiqiao Town. The croplands in Xiaobengbu Town and Wuxiaojie Town along Huaihe River mainly belong to the third and fourth gradation. The evaluation results of fertility level reflected accurately the status of local farmlands, which will provide the basis for the assessment of the soil fertility level of Huaihe River valley.