土壤溶解性有机碳四种测定方法的对比和转换
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湖南农业大学资源环境学院,湖南农业大学资源环境学院,湖南农业大学资源环境学院,亚热带农业生态过程重点实验室中国科学院亚热带农业生态研究所,湖南农业大学资源环境学院

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S153;X142

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国家自然科学基金项目(41571234、31100381)和湖南省自然科学基金项目(13JJ4066)资助


Comparison and Transform of Soil Dissolved Organic Carbon Measured by Four Methods
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College of Resources Environment,Hunan Agricultural University,College of Resources Environment,Hunan Agricultural University,College of Resources Environment,Hunan Agricultural University,Key Laboratory of Agro-Ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,College of Resources Environment,Hunan Agricultural University

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    摘要:

    针对土壤溶解性有机碳(DOC)不同测定方法之间可比性较差的问题,应用TOC仪法、容量法、紫外分光光度法和比色法分别测定了中亚热带丘陵山地6个土属共46个新鲜土壤样品的DOC含量。结果表明:参照TOC仪法测得的DOC数据,容量法一致性地低估20% ~ 67%;比色法测定DOC含量较低的底土时(<200 mg/kg)仅低估7% ~ 27%,但在分析DOC含量较高的表土(>600 mg/kg)时最不敏感,低估达53% ~ 93%;紫外分光光度法在DOC含量较高时也存在一定的低估,但在DOC含量较低时高估65% ~ 189%。4种方法测得的DOC数据均呈极显著正相关关系(P<0.01)。统计分析获得的线性或指数方程可应用于将容量法、紫外分光光度法和比色法的测定结果向TOC仪法的数据转换,而且有必要针对表土和底土使用不同的转换方程或参数,这些经验转换方程的建立有助于增强不同研究结果之间DOC数据的可比性,也有利于推动土壤DOC测定标准的完善。

    Abstract:

    Soil dissolved organic carbon (DOC) is essential for soil fertility and environmental health. Currently several different methods are available to measure soil DOC, which lead to poor comparability for soil DOC levels. Here, we used four methods, i.e., total organic carbon(TOC) analyzer method, volumetry method, ultraviolet absorption method, and colorimetric method, to determine DOC concentration in fresh soil extracts. In total 46 fresh soil samples from profiles which were belonged to 6 soil genuses developed in hills and mountains in mid-subtropical region were collected. Results showed that comparing with DOC data from TOC analyzer, soil DOC concentrations from volumetry and colorimetric methods were underestimated by 20%–67% and 7%–93%, respectively. UV absorption method also underestimated for topsoils but overestimated by 65%–189% for subsoils. Positive significant correlations were existed among DOC data from these four methods (P<0.01). Linear or non-linear equations were developed to transform DOC data from volumetry, ultraviolet absorption, and colorimetric methods into that from TOC analyzer, and it is necessary to apply different equations and parameters for topsoils and subsoils. These empirical equations and parameters are useful in enhancing the comparability of soil DOC data from different studies and improving the standard of soil DOC measurement.

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盛 浩,宋迪思,王翠红,周 萍,张杨珠.土壤溶解性有机碳四种测定方法的对比和转换[J].土壤,2015,47(6):1049-1053. SHENG Hao, SONG Di-si, WANG Cui-hong, ZHOU Ping, ZHANG Yang-zhu. Comparison and Transform of Soil Dissolved Organic Carbon Measured by Four Methods[J]. Soils,2015,47(6):1049-1053

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  • 收稿日期:2014-12-29
  • 最后修改日期:2015-02-01
  • 录用日期:2015-03-06
  • 在线发布日期: 2015-11-23
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