Climate change significantly affects the growth and nutrition accumulation of the rice. The objective of this research was to investigate the effects of CO2 enrichment and warming on the content, accumulation and distribution of C and N in rice plant. The treatments included the enrichment of atmospheric CO2 concentration up to 500 mmol/mol (CE), warming of canopy air with temperature increment of 2℃ (WA), and combined CO2 enrichment and warming (CW) in comparison to ambient condition (CK). The results showed that: 1) CE treatment significantly decreased N concentration in rice plant and C and N distribution in rice spike, while elevated CO2 significantly increased C concentration and C and N accumulation in rice plant. The treatment also increased C and N distribution in rice stems. 2)WA treatment significantly increased N concentration in rice plant and C concentration in the leaf at jointing stage and mature stage. Meanwhile, WA treatment significantly decreased C concentration in rice grain at flowing stage and mature stage. WA treatment significantly increased C accumulation at filling stage and flowing stage and also significantly increased N accumulation at jointing stage. WA treatment also increased C distribution in rice stems at flowing and filling stage and decreased C and N distribution in rice grain at mature stage. 3) CW treatment significantly increased C concentration, but significantly decreased N concentration in rice plant at filling stage. The treatment also decreased C and N distribution in rice grain, but increased the distribution in stems at mature stage.