The main objective of the present study was to investigate the amendments to remediate manganese pollution soil combined hyperaccumulator plants. By using soil incubation experiment, 2.5% and 5% of sepiolite (S2.5, S5.0) and zeolite (Z2.5, Z5.0) were added to study the effects on Mn hyperaccumulator Polygonum pubescens Blume’s growth, Mn absorption and antioxidant enzyme activities. The results showed that sepiolite and zeolite significantly increased Mn in the leaves of P. pubescens (P<0.05). However, sepiolite and zeolite also decreased the plant weight, the contents of chlorophyll a and b in the leaves of P. pubescens . S2.5 and Z5.0 treatments increased the height of P. pubescens by 15% and 16%, respectively, compared to the control group, while Z2.5 and Z5.0 decreased the height of P. pubescens by 40.05% and 35.58% respectively. In addition, sepiolite and zeolite significantly increased the contents of MDA and H₂O₂ (P<0.05) indicating the P. pubescens was under surpress. The content of superoxide radical (·O_2-) decreased 4.69%, 16.49% and 21.01% in S2.5, S5.0 and Z2.5 treatments, respectively. Furthermore, sepiolite and zeolite changed the activities of the antioxidant enzymes and the content of non-enzymes antioxidant. Sepiolite significantly increased the activity of superoxide dismutase (SOD) in the leaves, as indicated by the results that S2.5 and S5.0 treatments increased SOD activity by 14% and 15% respectively. The activities of peroxidase (POD) and catalase (CAT) also increased. The activities of POD were 4.63 and 4.23 times of the control in the S2.5 and S5.0 groups respectively. Z2.5 and Z5.0 treatments increased POD activities by 4.71 times and 4.74 times of the controls. However, both sepiolite and zeolite decreased the activity of ascorbate peroxidase (APX), –SH and GSH (P<0.05). These results demonstrate that sepiolite and zeolite have different impacts on the antioxidant enzymes in P. pubescens .