Bacterial-feeding nematodes can influence the function of soil ecosystems by altering bacterial activity and community structure. There is a feeding preference when bacterial-feeding nematodes confront towards different bacteria. A “cafeteria” diet experiment was set up to study the feeding behavior of Acrobeloides sp. and Mesorhabditis sp. to the living bacteria, killed bacteria and bacterial secretions of five bacteria in order to help us understand better the interactions between bacterial-feeding nematodes and bacterial community structure in soil ecosystems. The results demonstrated that the feeding preferences of Acrobeloides sp. and Mesorhabditis sp. to five living bacteria were similar. The two nematodes both preferred G-bacteria and the feeding preference order was P. fluorescens > E. coli > Variovorax sp.> B. amyloliquefaciens > B. megaterium. Acrobeloides sp.did not show preference to the five bacterial secretions which led to the feeding preference of Acrobeloides sp. to living bacteria and killed bacteria were similar. In contrast, there were big differences in feeding preference of Mesorhabditis sp. to living bacteria and killed bacteria, this may be related to the feeding preference of Mesorhabditis sp. to different bacterial secretions. However, the mechanism of bacterial secretions on feeding preference of Mesorhabditis sp. needs further study.