In this study, soils were treated with 0, 100 and 1 000 g/kg pristine graphene and graphene oxide for up to 56 days, respectively. Treatment effects on urease and catalase activity, microbial communities were evaluated. Microbial community sizes were evaluated by using both Biolog-eco plate and DGGE method. The results showed that the introduction of graphene, as either pristine graphene or graphene oxide, had little impact on urease and catalase activities after cultivation for 0, 7, 14 and 56 days. AWCD values of red soil and fluvo-aquic soil treated with graphene and graphene oxides showed no differences as compared with control. Only DGGE results showed a little changes of bacterium genera in fluvo-aquic soil treated with graphene oxide.