Reductive soil disinfestations (RSD), namely amending organic materials and mulching or flooding to create strong reductive status, has been widely applied to improve degraded soils, but the removal efficiency of sulfate (SO₄^2-) in RSD is low. To investigate the effect of liming on SO₄^2- removal during RSD treatment, a SO₄^2- accumulated (939 mg/kg) vegetable soil was treated by five treatments: control (flooding only, noted as CK), flooding + alfalfa (RSD₀) and RSD₀ + lime at three application rates, which adjusted soil pH to 6.7, 7.7 and 8.4, noted as RSD₁, RSD₂ and RSD₃, respectively. The results showed that SO₄^2- content in CK treatment was as high as 691 mg/kg, and was significant higher than those in RSD treatments after the incubation. In RSD treatments, SO₄^2- contents decreased with the increase of pH. But, other sulfur (S) forms increased significantly, which indicated that disappeared SO₄^2– was mainly transformed into other S forms. Total S contents in RSD+liming treatments were higher than that in RSD₀ treatment. The results indicates that liming stimulates the conversion of SO₄^2- into calcium sulfate, which effectively decreases SO₄^2- content but cannot decrease total S content.