A strip ultra-deep subsoiling method (DC) was introduced to investigate its effect on soil environment and tobacco yield and quality. Results showed that DC treatment significantly improved soil physical and chemical properties compared with the conventional treatment (CK). Soil moisture content and total porosity increased by 8.54% and 7.46%, while bulk density decreased by 7.99%. Moreover, soil pH increased by 0.71 unit (P<0.05). DC treatment increased high-class tobacco leave yield by 134 kg/hm² compared with CK. At the same time, DC treatment decreased nicotine and total nitrogen content by 10.81% and 27.04%, while increased total potassium and reducing sugar contents by 4.35% and 10.24%, respectively, suggesting DC treatment can enhance the quality of tobacco leaves, it is suitable and practical for upland tobacco production in South Anhui and other similar regions in China as well as for other similar crops.