In this study, the cow manure vermicompost, peat, perlite, and vermiculite were mixed with different proportions to select the best substrate for strawberry seedlings through the analysis of strawberry growth. The results showed that the substrates were general suitable for strawberry growth. Compared with the conventional substrate, the substrates mixed with different portions of vermicompost improved the quality of maternal and child strawberry seedlings (except the treatment with peat replaced by the vermicompost entirely). With the increasing proportions of vermicompost, the growth of strawberry seedlings firstly increased and then decreased. In all treatments, one-third vermicompost in substrate material volume of seedling substrate had the best effect on strawberry seedlings in general, compared with the conventional substrate, this treatment significantly increased the quality of strawberry maternal and child seedlings, and improved the plant height, biomass, root morphology index, SPAD value and peroxidase activity of the maternal strawberry seedlings, the leaf area, petiole length and petiole coarse of the child strawberry seedlings, etc. So it can be recommended for the actual production of strawberry seedling.