A field experiment was conducted to study the effects of inorganic minerals and biochar amendments applied in various combinations on the yield and quality of flue-cured tobacco and soil properties of slope upland in South Anhui, China. The results showed that applications of various amendments prepared from 3 kinds of inorganic mineral materials (T20, G20, diatomite) and 2 kinds of biochars (bamboo and rice husk) reduced soil bulk density by 0.27–0.34 g/cm³, increased soil moisture by 11.6%–55.6% and pH by 0.45–0.93 units, and thus increased the flue-cured tobacco yield by 26–397 kg/hm². Applications of the amendments also decreased nicotine and total nitrogen contents, increased the reducing sugar content and the ratio of sugar to nicotine, and thus improved tobacco quality. The results presented in this study can provide useful references for soil amelioration, and increases in yield and quality of tobacco growing in the soil in South Anhui.