The effects of sulphur powder application, water-controlled irrigation and fertilization on chlorophyll fluorescence parameters in leaves of blueberry were investigated using orthogonal design. The main objective was to identify the order of the three factors and the best level on PSⅡ reaction center function in leaves of blueberry. The results showed that PSⅡ reaction center function in leaves of blueberry was affected by the three factors obviously, and the fluorescence parameters under different treatments were significantly better than that of control. Range analysis and partial least squares regression showed that the effects of the three factors on PSⅡ reaction center function in leaves of blueberry followed the order: water-controlled irrigation> application of sulphur powder>fertilization. In addition, quadratic term coefficient of partial least squares regression equation showed that the three factors had the most suitable range. The opposite effect occurred out of this suitable range. Comprehensive analysis suggested that the best agronomic measures in the Greater hinggan mountains area of China are: 70–80 g/m² of sulfur powder, soil relative water content at 45%–60%, potassium sulphate fertilizer of 5~7.5 g/plant.