Phenanthrene and pyrene were used as representative polycyclic aromatic hydrocarbons (PAHs) to investigate their removal efficiencies from soil slurry by mixed microbes of thermophilic bacteria and PAHs degrading bacteria and their influencing factors of soil/water ratio, and the concentrations of glucose, starch and salicylic acid with indoor simulated experiments. The experiments were conducted at 40℃. At this temperature, the two kinds of microbes grow and reproduce quickly. The results showed that the removal rates of the two PAHs from soil slurry by the mixed microbes were significantly greater than theoe in control (P<0.01). The maximum removal rates of phenanthrene in a single day was 20%, and that of pyrene was 15.3%. With the progress of the reaction, the removal rates of phenanthrene and pyrener increased significantly, but the removal reaction rate gradually decreased. The half-life of phenanthrene was 1.8 d, and less than 4.9 d of pyrene. Therefore, the removal of phenanthrene was faster than that of pyrene. The most appropriate water/soil ratio for the removal of the PAHs by the mixed microbes in soil slurry was 2︰1, and the carbon source was glucose with TOC_glucose/TOC_PAHs ratio of 2︰1. The results presented in this study can provide theoretical basis and technical support for remediation of PAHs contaminated soil by mixed microbes.