通过室内泥浆体系模拟试验，研究了混合微生物菌群(嗜热菌和多环芳烃特异性降解菌)，在40℃条件下(两类微生物均能较快生长繁殖)，对泥浆体系中代表性多环芳烃菲、芘的去除效果及其影响因素(水土比，葡萄糖、淀粉、水杨酸及其浓度)。结果表明：泥浆体系中混合微生物菌群对多环芳烃的去除效果显著(P<0.01)，单日菲去除率最大可达20.0%，芘达15.3%。随着反应进程的进行，菲和芘的去除率提高，去除速率则逐步降低，菲的半衰期1.8 天小于芘4.9 天，因此菲的去除较芘更快。试验得到该泥浆体系中混合微生物菌群去除多环芳烃最合适的水土比为2︰1，碳源为葡萄糖，浓度TOC_葡萄糖：TOC_PAHs为2︰1。该研究结果可为泥浆体系中混合微生物菌群强化修复多环芳烃污染土壤的技术研发提供理论基础和技术支撑。

Phenanthrene and pyrene were used as representative polycyclic aromatic hydrocarbons (PAHs) to investigate their removal efficiencies from soil slurry by mixed microbes of thermophilic bacteria and PAHs degrading bacteria and their influencing factors of soil/water ratio, and the concentrations of glucose, starch and salicylic acid with indoor simulated experiments. The experiments were conducted at 40℃. At this temperature, the two kinds of microbes grow and reproduce quickly. The results showed that the removal rates of the two PAHs from soil slurry by the mixed microbes were significantly greater than theoe in control (P<0.01). The maximum removal rates of phenanthrene in a single day was 20%, and that of pyrene was 15.3%. With the progress of the reaction, the removal rates of phenanthrene and pyrener increased significantly, but the removal reaction rate gradually decreased. The half-life of phenanthrene was 1.8 d, and less than 4.9 d of pyrene. Therefore, the removal of phenanthrene was faster than that of pyrene. The most appropriate water/soil ratio for the removal of the PAHs by the mixed microbes in soil slurry was 2︰1, and the carbon source was glucose with TOC_glucose/TOC_PAHs ratio of 2︰1. The results presented in this study can provide theoretical basis and technical support for remediation of PAHs contaminated soil by mixed microbes.

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国家自然科学基金项目(41401345)资助。