Taking F you 498 rice variety as test material, a field experiment was carried out to study the effects of different ratios of controlled-release nitrogen fertilizer (CRNF) combined with urea (UR) on rice (Oryza sativa L.) yield, and nitrogen use efficiency. The results showed that, compared with the treatment of UR (T1), the treatments of combined with controlled-release nitrogen fertilizer (T2 – T6) increased the rice dry matter and yield for 3.7% – 13.9% and 1.4% – 13.4%, respectively. The dry matter and grain yield with combined treatments (T2 – T5) were 6.3% – 16.7% and 2.8% – 16.6% higher than CRNF only (T6). The nitrogen uptake of grain with 40% CRNF +60% UR was 24.2% higher than that of CRNF only, and the difference reached significant level (P<0.05). Meanwhile, the nitrogen apparent utilization efficiency (NAUE), nitrogen agronomic utilization (NAE), nitrogen physiological efficiency (NPE), nitrogen partial factor productivity (NPFP) were also in higher levels with the treatment of 40% CRNF +60% UR. Under experimental condition, 40% CRNF + 60% UR was the optimal treatment for high yield and nitrogen efficiency in rice, and could be popularized to practice.