Quinclorac is an effective herbicide commonly used in rice plantation. But Quinclorac residue in soil probably brings teratogenesis to some Quinclorac sensitive rotation crops, like Solanaceae plants, Leguminosae emblements. So the residue in soil should be accurately detected. Quinclorac residue in soil sample was extracted on a to-and-fro shaker for 2 hours with 50 ml extractant, which was the mixture of methanol and 0.05 mol/L borax solution (pH = 10) at the ration of 9:1 (V/V). The extracts was centrifuged and subsequently filtered. 25 ml filtrate was concentrated to about 1 ml and then diluted to 2 ml with methanol for high performance liquid chromatography (HPLC) determination. Content of Quinclorac in extracts was calculated according to external standard method by HPLC under optimized condition. Briefly, 1.0 ml/min methanol: 1% (V/V) acetic acid solution (55︰45, V/V) was selected as mobile phase to elute the target compound for ultraviolet (UV) detection at 238 nm wavelength. The Quinclorac detection limit of this method was 0.012 mg/kg; the recovery rates of different type soil samples lingered in the scope of 74.9% – 98.8% with precision being 6.0% (n=6). All demonstrate that the method meets the requirements of organic analysis. The method is relatively simple and easy to operate. As well as it can be used to analyze the low concentration of Quinclorac residue in soil sample.