Characteristics and correlation analyses of soil nutrients and enzyme activities in the middle reaches of Hui River Wetland National Nature Reserve were studied by using field sampling and laboratory analysis. The increase of distance from the Hui River and elevation gradient result in significant gradient changes of physical and chemical properties of soil in the riparian zone of the Hui River Wetland. The contents of total nitrogen (TN), total phosphorus (TP) and organic matter (SOM) increased first and then decreased from reed to herb zones, but soil moisture content (MC) continues to declined. The activities of alkaline phosphatase and urease showed increasing trends along the land succession of vegetation communities, and were significantly positive correlated with the values of TN, TP and SOM (P<0.01), but negatively to MC (P<0.01). The comprehensive analysis showed that soil enzyme activities in the riparian zone of Hui River Wetland are not only related to soil properties, but also closely related to hydrological conditions, micro topography and vegetation succession.