In order to disclose the interaction between forest and the grassland ecosystems with atmosphere in Kanas National Nature Reserve in Xinjiang and the spatial and temporal variation of soil respiration rate (SRR) as well as the influential factors, LI - 8150 soil carbon flux measurement system was used to continuously and automatically monitor daily soil respiration rates under the two vegetation ecosystems in the growing season. The results showed that the diurnal variation of SRR under both forest and grassland ecosystems in the growing season presented obvious unimodal type curve, the monthly maxima of SRR both appeared in July, maximum of SRR under forest ecosystem (2.43 μmol/(m²·s)) was significantly higher than that under grass ecosystem (1.55 μmol/(m²·s)). Monthly SRR under forest ecosystem was obviously higher than that under grass ecosystem. The peak and bottom of SRRs occurred at 17:00–18:00 and at 9:00–10:00, respectively. Minimum of SRRs occurred in June under grass ecosystem (0.35 μmol/(m²·s)) and in May under forest ecosystem (0.71 μmol/(m²·s)). SRR was higher during the day than in the evening. CO₂ was the main exhalant ingredient throughout the day. SRRs of woodland and grassland ecosystems had similar change trend and significant positive correlation with soil temperature in the growing season, but not with soil moisture content.