Alpine soil in the Tibetan Plateau is thought to be sensitive to global climate change, and characteristics of soil enzyme kinetic and thermodynamic are of great significance due to its crucial role in alpine soil ecological and biochemical processes. The activities of phosphomonoesterase(PME), β-glucosidase(BG), cellobiohydrolase(CBH) and β-N-Acetyl- glucosaminidase (NAG) in topsoil ((0-10 cm) of the alpine treeline in the western Sichuan were determined by microplate fluorimetric method. The results showed that the activities of the four studied enzymes increased with increasing of substrate concentration(0-0.28 mmol/L), and activity of PME, BG, CBH and NAG were 109.01, 52.53, 6.51 and 6.63 μmol/(g·h), respectively, when initial concentration of substrate was 0.28 mmol/L. The temperature sensitivity(Q₁₀ values) of PME, BG, CBH and NAG were reduced as temperature increased, and all the Q₁₀maximum values of four enzymes appeared at 10-20℃. In addition, NAG activity was increased with temperature elevation, while PME, BG and CBHactivities increased just before 25 ℃.Therefore, all the four soil enzymes are more sensitive to temperature elevation under lower temperature conditions(0-20℃) than higher temperature, and the soil ecological and biochemical processes related these enzymes maybe impacted significantly when alpine soil warming, especially in warm season.