The Ebinur Wetland Reserve was taken as the study area, the measured data of soil properties and TM/ ETM+images on Oct. 5 of 1990, Sept. 25 of 2000 and Sept. 13 of 2015 were used as the basic information. First, The feasible classification system of LUCC was setup, then the dynamic changes of land use and space-time evolution of landscape pattern were analyzed during the 25 years and the effects of land use changes on the evolution of landscape pattern were explored further and deeply. The results showed that: ① The dynamic degree of land use was 0.97% from 1990 to 2015, showed a decreasing trend, and the change of land use transformed from severe state to equilibrium state. From 1990 to 2000, although the areas of forest land and paddy fields increased, the areas of mild-slight saline land and extremely severe saline land obviously increased. From 2000 and 2015, the area of water decreased evidently while the area of desert sharply increased, and the areas of severe saline and extremely severe saline increased too. ② During the 25 years, great changes occurred in the landscape pattern. CONTAG decreased gradually, dispersity index increased sharply, ductility of the landscape declined, landscape fragmentation exacerbated. SHDI and SHEI showed a stable increasing trend, indicating the increase landscape heterogeneity. ③The change of land use influenced landscape pattern, increased landscape dispersity and evenness index while decreased CONTAG, landscape was firstly fragmentated and then turned better to optimized and stabilized, but generally the trend of salinization and desertification was still stern. The study proposes further an improvement scheme, which is significant to rational use and sustainable development of land resources in study area.