Yunyan 87 was taken as the test tobacco variety, a field experiment was conducted on a soil with 15 a continuous tobacco-cropping and serious bacterial wilt in order to study the effects of carbonized tobacco stem (tobacco biochar) on soil physiochemical properties, microbial population and tobacco growth for restoring continuous cropping tobacco soil, further understanding the ecological effects of biochar on tobacco production, and accelerating the comprehensive utilization of tobacco waste. The results showed that compared with the treatment of conventional fertilization (CK), the treatment of CK+1 500 kg/hm² tobacco biochar could improve well soil physiochemical properties and nutrient status, promote soil microbial abundance, stimulate the growth of tobacco and increase the yield and earnings of tobacco.