AMT is transmembrane protein mediated high-affinity ammonium uptake in plants. It has been reported that the expression of most genes encoding AMT protein depends on external nitrogen level and environmental condition, but the mechanism of expression regulation is unclear. The expression of OsAMT1;2 was greatly induced by nitrogen-deficiency. Therefore, the sequence of OsAMT1;2 promoter was cloned by using PCR from rice genome DNA and analyzed by using software and experimental analysis fusion GUS reporter gene. Software predicted results showed that the promoter region contained not only the TATA box and CAAT box, but also a variety of cis-elements of abiotic stress, biological stress, hormone and light response, such as Box-w1, MBS, MeJA, TATC and ERE. OsAMT1;2 promoter was deleted along with 5′ end to construct five different pAMT12-x-GUS expression vector, which subsequently were transformed into rice callus to establish different transgenic rice lines. The GUS activity of T1 generation plants showed that the shortest promoter fragment (-211 bp) could start the GUS expression, but GUS activity was low and the effect of nitrogen-deficiency was not significant. The GUS activity and inductive effect of nitrogen-deficiency significantly increased when –763 bp promoter fragment fusion with GUS, additionally the inductive effect no longer significantly enhanced with the increase of the promoter length. The cis-element of abiotic stress and hormones related lies between –211 bp and –763 bp, whether correlation between nitrogen regulation and cis-elements of abiotic stress and hormones remains to be further probed.