The spatial and temporal variation of soil organic matter(SOM) of tobacco-planting fields at field scale was comprehensively analyzed by using geostatistics method and GIS platform. In total 606 soil samples were collected from Ziyun Town of Xiangcheng County of Henan Province six times in successive two years, and the spatial distribution maps of SOM were edited and analyzed. The results indicated that CV of SOM during each period was small, ranging from 13.69% to 24.56%. The mean content of SOM during each period in a year decreased first and then increased, which was significantly lower in July than those in April and October. The maximal range of spatial correlation of SOM ranged from 26 m to 203 m. SOM showed medium spatial autocorrelation in April 2013 and July 2014, expressed strong spatial autocorrelation in other duration. In the study area, the stable high value of SOM was mainly distributed in the west area while the stable low value appeared in the middle area. The area of SOM stable with time was the largest, accounting for 90.89% of the total study area. The above results prove that spatial and temporal analysis can provide more efficient approach to quantify SOM with the help of time stable analysis and provide a theoretical basis for accurate and efficient fertilization and field management.