In order to explore the characteristics of nitrogen mineralization in tobacco planting soil and its relationship with temperature and water, effects of the soil temperature (15, 28, 37 ℃) and the relative water content (50%, 65%, 80% field capacity) on nitrogen mineralization of tobacco growing soils in three typical aromatic producing areas of Dali, Bijie and Xuchang were studied in laboratory culture. The results showed that soil mineral nitrogen and nitrogen mineralization rate were closely related with temperature and moisture, sharing the increasing trend with temperature increasing. Under the same temperature, nitrogen mineralization was the highest for soil in Dali due to its highest SOM content, while nitrogen mineralization was the lowest for soil in Xuchang due to its lowest SOM content. The relationship between soil moisture and nitrogen mineralization were different in the three different ecological areas, the clayey soil in Bijie had the highest and lowest nitrogen mineralization and mineralization rate under 50% and 80% field capacity, respectively, meanwhile, the coarse-textured soils in Xuchang and Dali had the highest and lowest nitrogen mineralization under 65% and 50% field capacity , respectively. Based on the simulation of first-order kinetic equation, the potential mineralized nitrogen pools (N₀) of three soils increased with the increase of temperature. Generally, 28-37℃ was more suitable for nitrogen mineralization while temperature below 15℃ was unconducive to nitrogen mineralization. N₀ was the highest for soil in Dali but the lowest for soil in Xuchang while nitrogen mineralization rate constant (K) was the highest for soil in Dali. Soil moisture also had a certain impact on N₀ , and the above results showed that soil temperature and moisture have significant interaction on nitrogen mineralization and mineralization rate, thus, reasonable regulation of soil temperature and moisture can effectively control the dynamic changes of soil nitrogen mineralization in different ecological tobacco-planting areas.