This research examined the effects of the reduction of chemical fertilizer (RCF) combined with the application of microbial agents (EM and CY agents) on the yield and quality of cherry tomato and on soil properties. The results showed that the contents of soluble solids, protein and sugar under RCF + EM treatment were significantly greater than those under the CF treatment (31.05%, 27.82% and 62.73% higher, respectively, P<0.05). There was no significant difference in the yields between RCF + EM and CF treatments (P>0.05). Soil pH, contents of organic matter and available nutrients and microbial quantity under RCF + EM treatment were higher than those under CF treatment, among of which, soil pH, contents of available nitrogen and soil microbial biomass nitrogen were significantly higher (13.07%, 20.83% and 66.10% higher, respectively, P<0.05). Comparatively, the effects of RCF + EM treatment on cherry tomato were generally better than those of RCF + CY treatment. In conclusion, reduction of chemical fertilizer combined with the application of EM agents can not only promote the yield and quality of cherry tomato, but also improve soil fertility.