The contents of nitrogen (N) in soils and N concentrations in tea leaves and their relationship within 77 Tieguanyin tea (Camellia sinensis(L.) O. Kuntze cv. Tieguanyin) gardens in Anxi County of Fujian Province were investigated in this study. The results showed that soil total N contents in 0–20 cm layers were above the average grade with an average content of 1.13 g/kg, the proportion of soil samples with grade II (ordinary) and the higher grades reached 90.9%; while soil total N contents in 20-40 cm soil layers were below the average grade with an average content of 0.81 g/kg, the proportion of soil samples with grade II (ordinary) and the lower grade reached 77.9%. Soil alkaline nitrogen contents in 0-20 cm layers were abundant with an average of 105.42 mg/kg, the proportion of soil samples with grade II (ordinary) and the higher grades was 97.4%. The average of soil alkaline nitrogen contents in 20–40 cm layers was 75.02 mg/kg, the proportion of soil samples with grade II (ordinary) and the higher grades was 84.4%. Total N and alkaline nitrogen contents were higher in yellow soils, red soils, paddy soils and latosolic red soils while lower in the skeletal soils and fluvo-aquic soils. There was a significant positive correlation between the contents of soil total N and organic matter, between the contents of soil alkaline nitrogen and total nitrogen as well as organic matter. There was no significant correlation between N concentrations in tea leaves and soil total N and alkaline N contents. Further study is needed to determine whether the concentration of alkaline nitrogen in tea leaves could be used as a diagnostic index of soil N supplying capacity in Tieguanyin tea gardens and whether N concentration in Tieguanyin tea fresh leaves could be used as a nutrition diagnostic index during the picking stage. Attentions should be paid to deep placing N fertilizers, increasing organic manure and reducing available nitrogen fertilizer.