A total of 332 topsoil samples (0 – 20 cm) and 86 deep soil samples (> 150 cm) were collected in Jiexi County, Guangdong Province to study the characteristics of total selenium contents, spatial distribution and its influential factors by Pearson correlation analysis, regression analysis, geostatistical analysis and GIS technology. The results showed that the concentration of total selenium ranged from 0.16 to 1.61 mg/kg with a mean value of 0.53 mg/kg. The whole region was concentrated in the category of abundance of selenium content. Among the different types of parent materials, selenium contents were higher in soils developed from tuff, granite, and shale, while selenium concentrations were lower in soils developed from sandstone. Among the different land use types, the highest Se content was found in unused land, followed by agricultural arable land, and total selenium content was the lowest in construction land. Regression analysis and Pearson correlation analysis showed that soil total selenium content was significantly negatively correlated with pH, but significantly positively correlated with iron-aluminum oxide content, organic carbon content, and altitude. The parent material was the main influential factor of soil total selenium content in Jiexi County, while soil pH, organic carbon, iron and aluminum content, altitude, and land use type also had certain effects on the distribution and enrichment of soil total selenium content.