Iodine (I) and selenium (Se) are important trace elements for human body, and the lack of I and Se may lead to sub-health or a variety of diseases. Diet is a safe and efficient approach of Se and I supplement. However, there are insufficient studies now focusing on the interaction of I and Se on their uptake in plants. This study was to explore the interaction of I and Se on their uptake in tomato and lettuce and the capabilities of two crops in absorbing I and Se by adding potassium iodate and selenate (inorganic Se) with certain concentration gradients. The results showed that compared with lettuce, tomato had a stronger uptake capability for inorganic Se and potassium iodide (KI). There is no significant interaction on Se and I uptake in lettuce and tomato.