In this paper, 21 and 91 tobacco-planting fields with and without bacterial wilt disease were surveyed and studied, the results showed that bacterial wilt disease were occurred in the tobacco-planting fields in Luoshan, Pingqiao, Queshan, Biyang and Suiping, soil pH were mainly ranged from 5.5 to 6.5 (accounted for 84.75%); while no bacterial wilt disease was happenedin the tobacco-planting fields in the circumjacent regions including Tanghe, Zhengyang, Shangcai, Sheqi, Wuyang and Chenqu, soil pH were mainly ranged from 6.5 to 7.0 (accounted for 81.25%). Significant difference was found in soil pH values between the two regions with and without bacterial wilt disease, and soil pH 6.5 could be regarded as the occurrence threshold of tobacco bacterial wilt disease in Henan. There was extremely significantly positive linear correlation between soil pH and latitude (y=0.7375x–17.799，R²=0.386，P<0.01), north latitude 33° could be regarded as the geographic boundary of soil pH 6.5, and bacterial wilt disease is less likely occur in the north of this boundary.