In order to understand selenium content and characteristics of selenium enrichment of wild Dipsacus asperoides in Enshi of Hubei Province, samples of Dipsacus asperoides and their homologous soils were collected from eight different regions. Hydride generation atomic fluorescence spectrometric method was used to measure selenium contents in roots, stems, leaves and flowers of Dipsacus asperoides, and in soil samples. The ranges of selenium contents in roots, stems, leaves and flowers of Dipsacus asperoides and in soil samples were 0.029 - 94.87, 0.008 - 39.45, 0.040 - 123.14, 0.026 - 143.94 and 0.6 - 277.3 mg/kg, respectively. Significant linear correlation were found between selenium contents in roots, stems, leaves and flowers of Dipsacus asperoides and in soil samples. Selenium bio-concentration factor and transport factor of Dipsacus asperoides were 0.012 - 0.727 and 0.279 – 2.200, respectively. The two coefficients were the lowest in stems. Based on selenium transport factor, the samples of Dipsacus asperoides were divided into two types: Leaf enrichment (LE) which was in low soil selenium content and flower enrichment (FE) which was in high soil selenium content. The results revealed that Dipsacus asperoides is a plant species of selenium enrichment because it can grow normally in soil with high selenium content and has strong capability of selenium enrichment. This study has practical significance to breed and develop Dipsacus asperoides in selenium enriched environment.