Abstract:In this study, a field experiment was conducted in Chaohu Lake Basin with rice variety "Nangjing 9108" as the test material to investigate rice yield and selenium (Se) uptake, accumulation, translocation and distribution in rice under different Se supplying levels (0, 20, 40 and 60 g/hm2) with single and two-time fertilization. The results showed that under single Se fertilization, there was no significant increase in rice yield between different Se supplying levels. Se supplying significantly increased Se contents in stems, leaves and panicles of rice, but they also showed the trend of increasing and decreasing with time. Se contents in rice, brown rice and milled rice were positively correlated with Se supplying levels, and the average Se contents in rice, brown rice and milled rice with Se supplying were 0.279, 0.150 and 0.055 mg/kg, respectively, which was 309%, 142% and 333% higher than that of CK (no Se fertilizer). Se accumulation in rice shoots was positively correlated with Se supplying level, and Se transport coefficient and harvest index significantly decreased, although Se apparent utilization rate was increased with the increase of Se supplying levels. Two-time fertilization increased significantly rice yield than single fertilization, and rice yield increased by 15% compared with CK. Compared with single fertilization, two-time fertilization increased Se contents in rice husk and rice bran while not in milled rice. The harvest index and transporting coefficient of Se fertilizer significantly decreased with higher apparent utilization rate of Se fertilizer, which indicated more Se accumulated in straws. In conclusion, exogenous Se can increase Se content in rice and makes it meet the national standard of selenium-rich rice, however, a significant amount of Se is accumulated in hull, chaff and straw, which is need to be used fully.