Abstract:In this study, earthworm manure, pig manure, chicken manure, straw manure, rice bran, peat, and peanut shell powder were used as carriers. The carbendazim-degrading bacteria Rhodococcus qingshengii djl-6 and the acetamiprid-degrading bacteria Pigmentiphaga sp. D-2 were used as materials. After being inoculated the strains djl-6 and D-2, all carriers could promote the growth of the tested strains. When earthworm manure was used as the carrier, the effective release rates of strains djl-6 and D-2 were the highest, reaching 100.40% and 82.03% respectively. When stored for 120 d, the number of viable bacteria in each carrier except peanut shell powder was higher than 107 cfu/g. The live bacterial count of djl-6 bacterial agent using earthworm manure as a carrier reached 7.00×108 cfu/g, and the live bacterial count of D-2 bacterial agent using pig manure as a carrier reached 4.29×108 cfu/g. After the solid bacterial agent was stored for 30 d, the soil pesticide residue degradation test was performed. The djl-6 bacteria agent with earthworm manure as a carrier degraded 94.30% of carbendazim at 5 mg/kg, and the D-2 bacteria with earthworm manure as a carrier degraded 81.87% of carbendazim at 10 mg/kg. The results of this study indicated that the solid pesticide residue degrading bacterial agent using earthworm manure as a carrier has a high number of live bacteria and a long shelf life, and has a good application prospect.