The effects of biochar addition (at rate of 1%) and field aging (for 3 years) on antibiotic transport were investigated in calcareous purple soil of farmland, which is distributed widely in the hilly areas of the upper Yangtze River. The sorption and transport of sulfadiazine and sulfamethazine in the soils were studied by batch equilibrium method and breakthrough experiments with repacked soil columns, respectively. The results showed that the isotherm adsorption data of both antibiotics were fitted better by the Freundlich equation. Biochar application improved the adsorption of both antibiotics to the soils, with the values of the Freundlich adsorption capacity (K_f) following the order: 1% biochar amended soil after 3 years of ageing > 1% fresh biochar amended soil > control soil. The peak relative concentrations of both antibiotics in the column effluents followed the order: 1% biochar amended soil after 3 years of ageing < 1% fresh biochar amended soil < control soil, indicating that biochar application could effectively reduce the leaching of the antibiotics. Field ageing enhanced the retention and transport of the two antibiotics in the soils, probably as the result of the observed increase in soil pH, and such effects were more pronounced for sulfamethazine than for sulfadiazine.