Construction and application of tobacco planting soil quality evaluation system in Yunnan (2017yn06), science and technology project of Hongta Tobacco (Group) Co., Ltd. (s-6016010)
应用定量PCR技术和高通量测序技术研究施用有机肥（YJ）、有机无机肥（YW）和无机肥（WJ）3 a后土壤理化性质和细菌群落结构、数量和功能的变化。结果显示不同施肥处理下，土壤理化性质发生改变，其中WJ和YJ处理降低了土壤pH，但YJ处理能显著提升土壤有机质含量，而YW处理在维持土壤pH的同时提高土壤速效养分和有机质含量。土壤细菌数量、群落结构和功能也相应地发生变化，YJ和YW处理土壤中的细菌16S rRNA基因拷贝数显著高于WJ，YW处理的土壤细菌群落alpha多样性最高。主坐标分析和ANOSIM检验进一步表明，影响土壤细菌群落结构的最主要因素是土壤pH。此外，施肥3 a后土壤中部分益生菌相对丰度也发生明显的变化，YW处理土壤中Gemmatimonadaceae、Micrococcaceae和Haliangiaceae等植物促生菌的丰度显著较高，YJ处理土壤中Intrasporangiaceae、Xanthomonadaceae、Chitinophagaceae、Burkholderiaceae等具有固氮或生防功能的细菌丰度较高，YW和YJ处理均增加了固氮菌Rhizobiaceae的丰度。细菌群落结构的改变进一步影响了细菌群落的功能，YW处理显著增加了土壤细菌群落的硝化功能。本研究结果可为烟叶生产中合理施肥提供科学依据。
In this study, the changes in soil bacterial community structure, quantity and function were examined by a 3 a fertilization experiment using quantitative PCR and high-throughput sequencing techniques, which included three treatments:organic fertilizer (YJ), inorganic fertilizer (WJ), and the combined application of organic and inorganic fertilizer (YW). The results showed fertilization treatments caused the changes in soil physiochemical properties, specifically, WJ and YJ decreased soil pH, YJ significantly increased soil organic matter content, YW increased available nutrients and soil organic matter content while maintaining soil pH. Additionally, the quantity, community structure and function of soil bacteria also changed, the copy numbers of bacteria 16S rRNA gene in YJ and YW soils were significantly higher than in WJ soil, the alpha diversity of soil bacteria community in YW soil was the highest. Principal coordinate analysis and ANOSIM test further revealed soil pH had greatest influence on soil bacterial community structure. Moreover, some bacterial groups were obviously enriched, YW soil had significantly higher abundances of plant growth-promoting bacteria such as Gemmatimonadaceae, Micrococcaceae and Haliangiaceae. YJ soil had higher abundance of bacteria with nitrogen fixation or biocontrol functions such as Intrasporangiaceae, Xanthomonadaceae, Chitinophagaceae and Burkholderiaceae. Furthermore, YW and YJ increased the abundance of nitrogen-fixing bacteria Rhizobiaceae. Notably, the change in bacterial community structure may further influence bacterial community function, especially, YW can greatly enhance nitrification function of bacterial community. The above findings provide a scientific basis for rational fertilization in tobacco production.
王珏,杜琴,彭双,林先贵,王一明,李军营,戴勋,谢新乔.不同施肥处理对植烟土壤细菌群落的影响[J].土壤,2021,53(5):998-1007. WANG Jue, DU Qin, PENG Shuang, LIN Xiangui, WANG Yiming, LI Junying, DAI Xun, XIE Xinqiao. Effects of Different Fertilization Treatments on Bacterial Community in Tobacco-planting Soil[J]. Soils,2021,53(5):998-1007复制