一种通用型土壤有效元素高效提取方法及应用
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S153.1

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国家重点研发计划项目(2019YFC1803403,2017YFF0108201)、国家自然科学基金项目(41771275)和江苏高校优势学科建设工程项目(PAPD)资助。


A Universal Method for Efficient Extraction of Soil Available Elements and Its Application
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    摘要:

    Mehlich 3(M3)方法可同时提取土壤中P、K、Ca、Mg、Al、Fe、Zn、Mn、Cu等多种元素的有效态,也同时适用于钙质土、火山灰土、高度风化土等各类土壤。但是由于硝酸铵(NH4NO3)为管制化学品,不易获取,应用M3方法测定土壤有效元素含量时受到极大限制。因此,本研究用另一种易获取的惰性电解质NH4Cl替代NH4NO3,评价NH4Cl替代NH4NO3的M3法(NH4Cl-M3法)适用性。Pearson相关分析结果表明,用NH4Cl-M3法测得的30种土壤有效P、速效K含量与M3法测定结果呈极显著正相关,其决定系数(R2)分别为0.99(P=2.31×10-55n=60)和0.98(P=1.52×10-49n=60)。同时,NH4Cl-M3法和M3法测定的有效Ca、Mg、Al、Fe、Zn、Mn、Cu含量极显著相关,R2值分别为0.98,1.00,0.99,0.96,0.99,0.95和0.94。NH4Cl-M3-P与酸性、中性和碱性土壤的Bray-P和Olsen-P极显著相关,R2分别为0.97和0.91(P=1.42×10-15、1.00×10-21n=20、40)。同时,利用NH4Cl-M3法测定酸性改良土壤有效P含量,发现其得到显著提高。因此,NH4Cl-M3法可作为测定各种土壤,以及改良土壤中有效P、速效K和其他金属元素有效态含量的通用方法。

    Abstract:

    Mehlich3 (M3) method can simultaneously determine the contents of soil available P, K, Ca, Mg, Al, Fe, Zn, Mn and Cu, and is feasible for calcareous, andosol and highly weathered soils. However, it is hard to get NH4NO3 used in M3 method due to its explosibility, therefore, it is necessary to find other substances to replace NH4NO3. In this paper, M3 method was modified by using indifferent-electrolyte ammonium chloride (NH4Cl) to replace NH4NO3 (NH4Cl-M3 method) to determine available elements in 30 topsoils. Pearson correlation analysis showed available P and K contents in the tested 30 topsoils determined by the two methods were significantly correlated, with determination coefficients (R2) of 0.99 (P=2.31×10-55, n=60) and 0.98 (P=1.52×10-49, n=60), respectively. Meanwhile, significant correlations were also found for available Ca, Mg, Al, Fe, Zn, Mn and Cu contents determined by the two methods, with R2 values of 0.98, 1.00, 0.99, 0.96, 0.99, 0.95 and 0.94, respectively. Bray-P and Olsen-P of the above acid neutral and alkaline topsoils determined by the two methods were also significantly correlated, with R2 values of 0.97 and 0.91 (P=1.42×10-15 and 1.00×10-21), respectively. Soil available P content determined by NH4Cl-M3 method was significantly promoted than M3 method. Therefore, NH4Cl-M3 method can be an alternative method to determine available P, K and other elements in calcareous and highly weathered soils as well as the amended soils.

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刘慧,董颖,王玉军,俞元春,姜军,徐仁扣.一种通用型土壤有效元素高效提取方法及应用[J].土壤,2021,53(5):1040-1047. LIU Hui, DONG Ying, WANG Yujun, YU Yuanchun, JIANG Jun, XU Renkou. A Universal Method for Efficient Extraction of Soil Available Elements and Its Application[J]. Soils,2021,53(5):1040-1047

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