In order to study the available content of soil nutrient elements and their influencing factors in Qingzhou City, Shandong Province, 390 topsoil samples were systematically collected and analyzed. The total and available contents of soil nutrient elements such as N, P, K, Cu, Zn, Mo and B, as well as pH and organic matter content were obtained, and the correlation and difference were analyzed. The results showed that the available contents of soil Mo, B elements in the study area were low, and the overall performance was lack and relatively lack. The background value of N element available content was slightly higher than the critical value, and its distribution was irregular. The variation coefficients of P, K, Cu, Zn elements available contents were greater than 100%, showed a spatial distribution trend of low west and high east. The correlation analysis showed that the total contents of N, P, K, Cu, Zn, B elements were significantly positively correlated with available contents, and the available contents of the 7 elements tested were significantly negatively correlated with pH. In addition, the available contents of N, K, Zn, B elements had a significant positive correlation with organic matter content. In different parent materials, pH of Ordovician and Cambrian soil and organic matter content of Ordovician soil were significantly higher than the whole area, but the available contents of soil nutrient elements were generally lower in Ordovician soil. In different soil types, pH and organic matter content of coarse bone soil were obviously higher than the whole area, while the available contents of soil nutrient elements were generally lower. The available content of soil nutrient elements in the study area was not only closely related to the total content of elements, but also affected by soil pH and organic matter content.