In order to quantitatively evaluate the response of flue-cured tobacco yield and quality to the combined application of organic-inorganic fertilizers and the influencing factors at the national and regional scales, and provide the bases for scientific fertilization of flue-cured tobacco and improvement of production profit, through the cnki.net database, "flue-cured tobacco", "organic fertilizer" and "yield" were selected as the main keywords to retrieve journal literatures, inorganic fertilizer only was used as control, the response ratio was used as the effect size, and the literature integration analysis method was used in the study. Compared with single application of inorganic fertilizers, the average increase in E of flue-cured tobacco under the treatment of combined application of organic-inorganic fertilizers was 4.68%, and significantly increased the proportion of first-class tobacco by 13.55%. The combined application of organic-inorganic fertilizers did not reach significant effect on chloride ions (E=0.55%), but significantly reduced nicotine (E=-3.9%) and total nitrogen (E=-1.37%) while significantly increased reducing sugars (E=3.69%) and potassium ions (E=6.77%), and the combined application of organic-inorganic fertilizers could coordinate the chemical composition of tobacco leaves better than single application of chemical fertilizers, and reach the appropriate range of chemical composition of high-quality flue-cured tobacco. The results of subgroup classification analysis of the effect of flue-cured tobacco yield showed that trial time, planting density and planting tobacco region have significant influences on the yield effect of flue-cured tobacco, and with the time passage of the combined application of organic-inorganic fertilizers, the yield of flue-cured tobacco showed a linear increase trend; When the input amount of inorganic nitrogen > 60 kg/hm², the combined application of organic and inorganic fertilizers showed a significant positive effect on the flue-cured tobacco yield, and when the input amount of inorganic nitrogen >90 kg/hm², the yield increase reached the highest (E=4.1%). The combined application of organic and inorganic fertilizers did not have significant effect on flue-cured tobacco yield in fluvo-aquic soil, and the highest yield increase rate was obtained in purple soil (E=13.85%). On the whole, the combined application of organic and inorganic fertilizers has positive and obvious effects on the yield and quality of flue-cured tobacco and thus can be popularized.