In order to find out the influences of pH and soil organic matter (SOM) on the available contents of trace elements in tobacco-planting soil in central Henan Province, 191 typical topsoil samples (0-20 cm) were collected from six major tobacco-producing areas Jian'an, Yuzhou and Xiangcheng of Xuchang City as well as Chengqu, Linying and Wuyang of Luohe City, the contents of available trace elements (Cu, Fe, Mn, Zn and Mo) and SOM as well as pH values were determined, and the relationship between available trace elements with pH and SOM were analyzed by Pearson correlation and curve regression method. The results showed that the average contents of available Cu, Fe, Mn, Zn and Mo were 1.20, 49.24, 84.80, 1.71 and 0.02 mg/kg, respectively, Mn was generally sufficient, Mo extremely insufficient, and Cu and Zn insufficient in some soil samples. The average value of pH and SOM were 7.90 and 17.78 g/kg, respectively, which indicated pH was a bit higher while SOM was appropriate for tobacco-planting. Pearson analysis showed that there were quadratic function correlations between pH and SOM with trace elements, with the increase of pH, available Cu increased, Fe and Zn increased first and then decreased, and Mn and Mo decreased first and then increased; with the increase of organic matter, available Cu, Mn, Zn and Mo showed an increasing trend, while available Fe showed a trend of increasing first and then decreasing.