降解多菌灵和啶虫脒残留的复合菌剂研发及初步应用
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X172

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中央高校基本科研业务费项目(KYZZ2022001)和江苏省农业科技自主创新资金项目(CX(18)1005)资助。


Development and Preliminary Application of Compound Bacterial Agent for Degrading Carbendazim and Acetamiprid Residues
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    摘要:

    针对菜田土壤中多菌灵和啶虫脒的残留问题,以多菌灵降解菌株Rhodococcus qingshengii djl-6和啶虫脒降解菌株Pigmentiphaga D-2作为材料,进行降解菌剂的复配,研究了使用复合降解菌剂对复合农药残留土壤的修复效果及微生态效应。研究结果表明:①复配降解菌株djl-6与D-2的最适体积比为5∶3,复合降解菌剂在3 d内对无机盐培养基中50 μg/ml的多菌灵降解率为87.14%,对50 μg/ml的啶虫脒降解率为96.10%。②初始接种量为7%时,复合降解菌剂可在6 d内将复合农药污染土壤中5 mg/kg多菌灵降解74.40%,将5 mg/kg啶虫脒降解95.87%。土壤含水率为25%时,复合降解菌剂对复合农药污染土壤中5 mg/kg多菌灵的降解率为80.80%,对5 mg/kg啶虫脒的降解率为97.87%。③复合农药污染土壤中10 mg/kg多菌灵和10 mg/kg啶虫脒即可对小青菜的生长产生明显的药害,小青菜生长24 d时根长仅为空白对照的66.56%、茎叶长为58.35%、鲜重为45.13%。在7%的接种量条件下,复合降解菌剂可解除药害,使小青菜的生长指标恢复至对照水平。④多菌灵和啶虫脒残留造成土壤细菌群落的Observed species指数、Shannon指数、Chao1指数和ACE指数降低,均与浓度呈正相关,接入复合降解菌剂后各指数均提高。可见,该复合降解菌剂具有较好的应用前景。

    Abstract:

    In order to solve carbendazim and acetamiprid residues in vegetable soil, carbendazim degradation strain Rhodococcus qingshengii djl-6 and acetamiprid degradation strain Pigmentiphaga D-2 were used as materials to study the remediation effect and microecological effect of compound degradation bacteria on compound pesticide residue soil. The results show that:1) The optimum volume ratio of compound degradation strain djl-6:D-2 is 5:3, and the degradation rates of carbendazim and acetamiprid are 85.46% and 94.36% for 50 μg/ml respectively within 3 days. 2) When the initial inoculation amount is 7%, the degradation rates of 5 mg/kg carbendazim and acetamiprid in compound pesticide contaminated soil are 74.40% and 95.87% respectively within 6 days. When soil water content is 25%, the degradation rates of 5 mg/kg carbendazim and 5 mg/kg acetamiprid in soil contaminated by compound pesticides are 80.80% and 97.87%, respectively. 3) Soil contaminated by 10 mg/kg carbendazim and 10 mg/kg acetamiprid (compound pesticides) cause obvious drug damage to the growth of green vegetables. After growing for 24 days, the root length, stem and leaf length and fresh weight of green vegetables are only 66.56%, 58.35% and 45.13% of those of the blank control. Under the condition of 7% inoculation, the compound degradation bacteria can relieve the drug damage, and the growth index of green vegetables is consistent with that of the control. 4) Carbendazim and acetamiprid residues have significant inhibitory effects on Observed species index, Shannon index, Chao1 index and ACE index of soil bacterial community, which are positively correlated with the concentrations of carbendazim and acetamiprid. In conclusion, the compound degradation bacterial agent has a broad application prospect.

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李锦涛,杨涵,陈洋,焦宸,汤浩宇,何健,黄星.降解多菌灵和啶虫脒残留的复合菌剂研发及初步应用[J].土壤,2022,54(3):646-652. LI Jintao, YANG Han, CHEN Yang, JIAO Chen, TANG Haoyu, HE Jian, HUANG Xing. Development and Preliminary Application of Compound Bacterial Agent for Degrading Carbendazim and Acetamiprid Residues[J]. Soils,2022,54(3):646-652

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