The rhizosphere soil in Mo-mining area was taken as the research object, and the influence of Mo-mining on soil glomalin, stoichiometry characteristics and their relationship were explored. The results showed that soil C and N contents in the mining area were 25.30 g/kg and 1.00 g/kg, respectively, which were significantly lower than those in the recovery and virgin areas. Soil C/N reached 29.36 in the mining area, which was 2.02 and 1.30 times of those in the recovery and virgin areas, respectively. Soil total glomalin (TG) contents were 2.81 and 3.64 mg/g in the recovery and virgin areas, respectively, while easily-extracted glomalin (EEG) contents were 1.22 and 2.02 mg/g, respectively, which were 2.08, 2.70 times and 1.97 and 3.26 times of TG and EEG in the mining area, respectively. In addition, the results of regression analysis showed that soil C and N contents had a significant effect on the secretion of glomalin-related soil protein (GRSP) by arbuscular mycorrhizal fungi. It can be seen that Mo-mining mainly has led to a large loss of soil C and N, and has a significant inhibitory effect on the secretion of GRSP by arbuscular mycorrhizal fungi.