稻田反硝化速率测定方法研究进展
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S154

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国家自然科学基金项目(41807083,41977097)和安徽省高校自然科学研究项目重点项目(KJ2019A0495)资助。


Methods for Measuring Denitrification in Flooded Rice Paddies: A Review
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    摘要:

    反硝化作用是淹水稻田肥料氮损失的主要途径之一。采用合适的反硝化测定方法是开展稻田反硝化作用研究的前提。然而,由于反硝化过程主要产物N2的大气背景值较高,以及反硝化作用具有高度时空异质性,淹水稻田反硝化作用损失氮量难以准确量化一直是阻碍科学评价稻田气态氮损失的关键难题。本文综述了研究稻田反硝化作用的4种方法(乙炔抑制法、15N同位素示踪法、密闭培养-氦气环境法和N2/Ar比值-膜进样质谱法),分析了这些方法各自的优缺点和适用性,并提出了稻田反硝化研究的参考建议,以期推动稻田反硝化的研究。

    Abstract:

    Denitrification is a microbially facilitated process where nitrate (NO3-) is reduced and ultimately produces dinitrogen(N2) through a series of intermediate nitrogen oxide products, i.e., nitrite, nitric oxide, nitrous oxide. Denitrification has long been considered as one of the major pathways of nitrogen (N) loss when N fertilizer is applied to flooded rice paddies. However, due to the high atmospheric background concentrations of N2, the main denitrification product, and the high spatio-temporal heterogeneity of denitrification, it is difficult to accurately quantify denitrification rates in flooded rice paddies, which hinders the scientific evaluation of N loss from paddies. The selection of appropriate methods for determination of denitrification is the basis for studying denitrification in paddies. In this paper, four approaches developed to measure denitrification in flooded rice paddies were reviewed, i.e., acetylene inhibition technique, 15N tracing method, helium environment-direct N2 quantification, and N2/Ar ratio-membrane inlet mass spectrometry, and the advantages, disadvantages and feasibility of these methods were discussed, and some suggestions were proposed in order to promote the future research of denitrification in paddies.

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李晓波,马兰,马舒坦,马倩倩,颜晓元.稻田反硝化速率测定方法研究进展[J].土壤,2021,53(6):1107-1114. LI Xiaobo, MA Lan, MA Shutan, MA Qianqian, YAN Xiaoyuan. Methods for Measuring Denitrification in Flooded Rice Paddies: A Review[J]. Soils,2021,53(6):1107-1114

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  • 收稿日期:2021-09-02
  • 最后修改日期:2021-10-11
  • 录用日期:2021-10-13
  • 在线发布日期: 2021-12-09
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