三种城市景观竹土壤微生物群落结构特征
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S154.36

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国家自然科学基金项目(31902108)和上海市科技兴农项目(2021-02-08-00-12-F00778)资助。


Soil Microbial Community Structures Under Three Urban Landscape Bamboo Forests
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    摘要:

    为探究城市景观竹林对土壤微生物群落的影响,选取上海辰山植物园种植的3种景观竹,利用Illumina MiSeq高通量测序结合土壤理化指标分析研究了不同景观竹林对土壤理化性质和微生物群落结构特征的影响。结果表明:不同竹林土壤全磷、有效磷、有机质、全氮、全钾含量和电导率存在显著差异(P<0.05);土壤细菌丰富度和多样性指数在毛竹林土壤中最高,在淡竹林土壤中最低;土壤细菌优势门为放线菌门(Actinobacteriota)、变形菌门(Proteobacteria)、酸杆菌门(Acidobacteriota)和绿弯菌门(Chloroflexi),真菌优势门为子囊菌门(Ascomycota),其中放线菌门和绿弯菌门在淡竹林土壤中的相对丰度最高,且显著高于其他竹林,而变形菌门、粘球菌门(Myxococcota)的相对丰度在毛竹林土壤中最高,土壤真菌优势门在不同竹林间的差异不显著。此外,土壤细菌群落结构和丰度受土壤pH、硝态氮、电导率、全磷、铵态氮、含水率、速效钾和微生物生物量碳影响显著,而真菌群落变化受全磷、电导率和全钾影响显著。

    Abstract:

    To explore the effects on soil microbial community, different urban landscape bamboo forests (Phyllostachys edulis, P.iridescens and P.glauca) were chosen in the Chenshan Shanghai Botanical Garden as the study area. Illumina MiSeq high-throughput sequencing technology and soil physicochemical indexes were used. The results showed that the contents of soil total phosphorus (TP), available phosphorus (AP), organic matter (OM), total nitrogen (TN), total potassium (TK) and electric conductivity (EC) were significantly different among the three bamboo forests. The Sobs and Shannon indexes of soil bacteria were highest under P.edulist and lowest under P.glauca. Actinobacteriota, Proteobacteria, Acidobacteriota and Chloroflexi were the dominant phyla of soil bacteria, while Ascomycota was the dominant soil fungi. Meanwhile, the relative abundance of Actinobacteriota and Chloroflexi were significantly higher under P.glauca, while the relative abundance of Proteobacteria and Myxococcota were the highest under P.edulis. However, there was no significant difference in the dominant phyla of soil fungi among the three bamboo forests. Meanwhile, the community composition of soil bacterial was significantly correlated with pH, nitrate nitrogen (NN), EC, TP, ammonium nitrogen (AN), moisture content (MC), AK and microbial biomass carbon (MBC), while the fungal community was significantly affected by TP, EC and TK.

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付浩,曾歆花,宋志琳,兰思仁,黄卫昌.三种城市景观竹土壤微生物群落结构特征[J].土壤,2022,54(6):1165-1174. FU Hao, ZENG Xinhua, SONG Zhilin, LAN Siren, HUANG Weichang. Soil Microbial Community Structures Under Three Urban Landscape Bamboo Forests[J]. Soils,2022,54(6):1165-1174

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  • 收稿日期:2022-02-12
  • 最后修改日期:2022-03-17
  • 录用日期:2022-03-29
  • 在线发布日期: 2022-12-26
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