Abstract:To explore the effects of combined full-rate straw incorporation (SW) and biogas slurry (BS) on soil water-stable aggregates and aggregated-associated organic carbon, lime concretion black soil in North China Plain was used as the research object. A field experiment was conducted with treatments including blank control (CK), SW alone, SW combined with low, middle and high amounts of BS, i.e., 450 m3/hm2(SW+LBS); 750 m3/hm2(SW+MBS), 1050 m3/hm2( SW+HBS). Soil samples in different layers (0-20 cm and 20-40 cm) were collected and water-stable aggregates and aggregate-associated organic carbon were analyzed. The results show that compared with CK, SW and BS significantly improve the mass proportion of water-stable macro-aggregates and aggregate stability in different layers. SW+MBS has the greatest mass proportions of water-stable macro-aggregates (WR0.25), mean weight diameter (MWD) and geometric mean diameter (GMD), but the lowest percentage of aggregate destruction (PAD) and fractal dimension (D). SW alone and SW combined BS significantly increase the contribution rate of macro-aggregate associated organic carbon in different layers, but decrease that of aggregates <0.25 mm. SW+MBS results in higher contribution rate of macro-aggregate associated organic carbon than other treatments. Therefore, SW combined appropriate BS is a promising approach for lime concretion black soil in this region by promoting water-stable large aggregate formation and aggregate stability, changing contents of aggregate-associated organic carbon and facilitating organic carbon distribution in large aggregates. The most obvious enhancement is obtained by SW+MBS.