Abstract:To explore the effects of long-term fertilization on soil peroxidase (POD)-like activity, desert soil and red soil were collected from the different fertilization in two long-term fertilization stations aiming to compare their POD-like activities using the colorimetric reaction of nanozymes. The results show that soils exhibit a classic catalytic activity to natural horseradish peroxidase (HRP). POD-like activity of desert soil and red soil varies in a range of 0.4-1.0 U/kg. Long-term fertilization significantly alters soil POD-like activity. Among them, soils in the long-term manure (M) fertilization have the highest of POD-like activity (up to 0.7 U/kg), which is 70% higher than unfertilized soils. The fertilization time has no significant effects on soil POD-like activity from long term no fertilization (CK) and chemical fertilization (NPK). Notably, there is a linear correlation (R2=0.71, p<0.01) between fertilization time and soil POD-like activity from long term organic fertilization (M and NPKM). High temperature sterilization alters soil POD-like activity, which is dependent on soil type and fertilization treatment. POD-like activity of red soil is relatively stable and not easily affected by environmental conditions, suggesting soil intrinsic POD-like activity rather than the absorption of extracellular enzymes on soils. In contrast, POD-like activities of soils in the NPK and CK treatments have a poorly stability and are easily affected by external environments. Pearson correlation indicates that soil POD-like activity is significantly correlated with dissolved silicon and magnesium. In conclusion, fertilization practices can alter soil intrinsic POD-like activity and then affect soil nutrient turnover.