土壤类过氧化物酶活性对长期施肥的响应
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国家自然科学基金项目(U22A20608, 41977271)资助。


Effects of Long-term Fertilization on Peroxidase-like Activities in Soils
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    摘要:

    为探究不同施肥处理对土壤类过氧化物酶活性的影响,以灰漠土和红壤长期定位试验站施肥处理土壤为研究对象,借助纳米矿物颗粒的类酶颜色反应原理,研究了不施肥(CK)、施用化肥(NPK)、施用化肥和有机肥(NPKM)和施用有机肥(M)土壤类过氧化物酶活性的变化。结果表明:与天然辣根过氧化物酶(HRP)相似,土壤也具有经典的催化颜色反应,灰漠土和红壤类过氧化物酶活性范围为0.4~1.0 U/kg。长期施肥显著改变了土壤类过氧化物酶活性,其中以M处理土壤类过氧化物酶活性为最高,可达0.7 U/kg,比CK处理增加了70% 以上。不同施肥年限下,CK和NPK处理土壤类过氧化物酶活性并无显著差异,而M和NPKM处理土壤类过氧化物酶活性则随施肥时间线性增加(R2=0.71,P<0.01)。高温灭菌处理后,土壤类过氧化物酶活性大多没有改变,表明其活性是土壤本身固有的氧化还原特性;但M处理红壤类过氧化物酶活性比CK和NPK处理红壤稳定性更高。皮尔森相关性分析表明,类过氧化物酶活性可能影响土壤可溶性矿质元素(如硅和镁)水平。因此,施肥措施可以通过改变土壤固有类过氧化物酶活性而影响养分周转。

    Abstract:

    To explore the effects of long-term fertilization on soil peroxidase (POD)-like activity, desert soil and red soil were collected from the different fertilization in two long-term fertilization stations aiming to compare their POD-like activities using the colorimetric reaction of nanozymes. The results show that soils exhibit a classic catalytic activity to natural horseradish peroxidase (HRP). POD-like activity of desert soil and red soil varies in a range of 0.4-1.0 U/kg. Long-term fertilization significantly alters soil POD-like activity. Among them, soils in the long-term manure (M) fertilization have the highest of POD-like activity (up to 0.7 U/kg), which is 70% higher than unfertilized soils. The fertilization time has no significant effects on soil POD-like activity from long term no fertilization (CK) and chemical fertilization (NPK). Notably, there is a linear correlation (R2=0.71, p<0.01) between fertilization time and soil POD-like activity from long term organic fertilization (M and NPKM). High temperature sterilization alters soil POD-like activity, which is dependent on soil type and fertilization treatment. POD-like activity of red soil is relatively stable and not easily affected by environmental conditions, suggesting soil intrinsic POD-like activity rather than the absorption of extracellular enzymes on soils. In contrast, POD-like activities of soils in the NPK and CK treatments have a poorly stability and are easily affected by external environments. Pearson correlation indicates that soil POD-like activity is significantly correlated with dissolved silicon and magnesium. In conclusion, fertilization practices can alter soil intrinsic POD-like activity and then affect soil nutrient turnover.

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王苗苗,李波,余光辉.土壤类过氧化物酶活性对长期施肥的响应[J].土壤,2023,55(1):104-110. WANG Miaomiao, LI Bo, YU Guanghui. Effects of Long-term Fertilization on Peroxidase-like Activities in Soils[J]. Soils,2023,55(1):104-110

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  • 收稿日期:2022-05-22
  • 最后修改日期:2022-06-20
  • 录用日期:2022-06-23
  • 在线发布日期: 2023-03-10
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