In order to provide the basis for predicting and controlling soil acidification and scientific application of medium and trace element fertilizers, the interannual variation characteristics of pH value, medium and trace elements in tobacco-growing soils of 4 888 samples in 5 years (2012-2020) in Kunming were analyzed. The results show that, between 2012 and 2020, 1) The average pH is between 6.0 and 6.4, mainly distributed in slightly acidic to neutral grades, the frequency of strongly acidic soils is increased while those of slightly acidic and neutral soils are decreased with the planting years; 2) The average variation of organic matter content is between 29.7 g/kg and 32.2 g/kg, decreased gradually since 2018; 3) The average value of exchangeable Mg²⁺ is ranged from 240.6 mg/kgto 270.7 mg/kg, which is abundant on the whole but tends to decrease with the increase of the planting years; 4) Available Zn is mainly distributed in very high grades, and available B and water-soluble Cl^- are mainly distributed in low and very low grades; 5) Except for pH, the spatial variability of organic matter, exchangeable Mg²⁺, available Zn, available B and water-soluble Cl^- are higher spatiotemporal heterogeneity in the 5 years. In conclusion, under the conventional fertilization management, pH, organic matter and exchangeable Mg²⁺ of tobacco-planting soil are generally suitable, but with a slow downward trend in recent years. Therefore, based on the level of conventional fertilization, the balance of soil exchangeable Mg²⁺ and water-soluble Cl^- should be maintained, but the application rate of organic fertilizer and B fertilizer should be increased while that of Zn fertilizer should be reduced.